Initial Results

Monitoring bacterial growth on different antibiotics concentrations allows us to infer what is the minimum inhibitory concentration (MIC) for each antibiotic type. Antibiotic concentration just below the MIC are a good starting point for the experimental evolution. In these examples, we defined the MIC as the concentration with a doubling time above 100 minutes. The initial concentrations chosen for the evolution experiment are marked in RED.

MICs and doubling times.jpg

Daily drug regimen

Daily observed generation time

Daily Results

Daily drug regimen

The drug dosing regimen appears below and can be updated by participating student classes (by 3pm, Israel time). The measured generation time calculated by the first 4hr of growth will be updated daily.

Observed doubling time

Final Results

Final project results include the minimal inhibitory concentrations, sequencing of selected genes, and full genome sequence of the winning super-bug.

Example (Sanger sequencing):

Mutations in the gyrase A (gyrA) gene are associated with resistance to Ciprofloxacin. Use Blast2Seq tool to align the DNA sequences of gyrA from the ancestor strain and the same gene from an evolved strain adapted Ciprofloxacin