Initial Results - determining initial drug sensitivity

Monitoring bacterial growth on different antibiotics concentrations allows us to infer what is the minimum inhibitory concentration (MIC) for each antibiotic type. Antibiotic concentration around the EC50 (the concentration of a drug that gives half-maximal response) are a good starting point for the experimental evolution. In these examples, we defined the EC50 as the concentrations the result in an optical density that is half of the optical density without the drug (after 16h of growth). The EC50 concentrations chosen for the evolution experiment are marked in RED.

EC50.png

Daily drug regimen


Daily observed optical density (16hr)


Daily Choice forms

The drug dosing regimen appears below and can be updated by participating student classes (by 4pm Eastern Standard Time). The relative optical density calculated by the first 18hr of growth will be updated daily.


Sequencing Results

Final project results include the minimal inhibitory concentrations, sequencing of selected genes, and full genome sequence of the winning super-bug.

Whole Genome sequencing 

Sanger sequencing: