Initial Results

Monitoring bacterial growth on different antibiotics concentrations allows us to infer what is the minimum inhibitory concentration (MIC) for each antibiotic type. Antibiotic concentration just below the MIC are a good starting point for the experimental evolution. In these examples, we defined the MIC as the concentration with a doubling time above 100 minutes. The initial concentrations chosen for the evolution experiment are marked in RED.

Daily drug regimen

Daily observed generation time

Daily Choice forms

The drug dosing regimen appears below and can be updated by participating student classes (by 4pm Eastern Standard Time). The relative optical density calculated by the first 18hr of growth will be updated daily.

Sequencing Results

Final project results include the minimal inhibitory concentrations, sequencing of selected genes, and full genome sequence of the winning super-bug.

Whole Genome sequencing 

Sanger sequencing:

Mutations in the gyrase A (gyrA) gene are associated with resistance to Ciprofloxacin. Mutations in the elongation factor (fusA) are associated with resistance against Kanamycin. Use Blast2Seq tool to align the DNA sequences of the ancestor strain and evolved strains to identify the mutations in these genes.